Assessing the Action of Phytoestrogens on Alveolar Bone Tissue of Female of Ovariectomized Rats with Induced Periodontal Disease
Carolina de Cassia Boamorte, Laís Fernanda Pasqualotto, Rose Meire Costa Brancalhão, Lucinéia de Fátima Chasko Ribeiro, Patricia Oehlmeyer Nassar, Sara Cristina Sagae, Carlos Augusto Nassar

Abstract
Osteoporosis can be a risk factor for periodontal disease. One of the alternatives for preventing bone loss under these conditions is hormone replacement therapy with isoflavone, a phyto-hormone similar to estrogen. The objective of this paper was to assess the action of isoflavone on alveolar bone tissue of female ovariectomized rats with induced periodontal disease. Sixty female rats were randomly divided into 6 groups: Control (CON); Ligature (LIG); Ovariectomy (OVX); Ovariectomy and Isoflavone (OVX+ISO); Ovariectomy and Ligature (OVX+LIG); Ovariectomy, Isoflavone and Ligature (OVX+ISO+LIG); all animals were treated with a standard diet and water ad libitum. Experimental periodontitis was induced when the animals were 70 days old and then submitted to ovariectomy surgery after 79 days. The rats were euthanized when they were 100 days old and the mandibles dissected for macroscopic, microscopic and radiographic analysis. The result of the analysis showed that the groups OVX+ISO and OVX+ISO+LIG presented less alveolar bone loss than the groups that did not receive isoflavone therapy (OVX+LIG and LIG) (p< 0.05). Therefore, isoflavone presents a protection effect on alveolar bone loss and progression of periodontal disease enhanced by osteoporosis.

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