Studies of Antimicrobial Properties of Different Leaf Extract of Tulsi (Ocimum tenuiflorum) against Human Pathogens
Gomathinayagam Subramanian, Brij B. Tewari, Rekha Gomathinayagam

Abstract
Objective: To study the antimicrobial activity of the different leaf extracts of Tulsi (Ocimum tenuiflorum ), also known as Ocimum sanctum, against three human pathogens Escherichia coli, Staphylococcus aureus and Candida albicans Methods: Different extracts (Ethanol, Methanol, Ethyl acetate and chloroform) of dried leaf of O. tensanctum were tested against three human pathogens strains such as Escherichia coli, Staphylococcus aureus and Candida albicans through the well diffusion and the poison plate method. The Minimum inhibitory concentration (MIC) values of the crude extract of the tested plant leaves were determined. Result: Both methods (well diffusion and poison plate) showed the strongest activity in methanol extract. Among four methanol extracts, they show more inhibition against in S. aureus than E. coli and C. albicans. Conclusion: The result showed methanol leaf extract inhibits S. aureus growth.

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